Genome-wide transcriptional response of Yersinia pestis to stressful conditions simulating phagolysosomal environments

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• 作者：Dongsheng Zhou ; Yanping Han ; Jingfu Qiu ; Long Qin ; Zhaobiao Guo ; Xiaoyi Wang ; Yajun Song ; Yafang Tan ; Zongmin Du ; Ruifu Yang
• 关键词：Y. pestis ; Microarray ; Transcriptional profiling ; Mg²⁺ limitation ; Polymyxin treatment ; Oxidative stress
• 刊名：Microbes and Infection
• 出版年：2006
• 出版时间：October 2006
• 年：2006
• 卷：8
• 期：12-13
• 页码：2669-2678
• 全文大小：165 K

文摘

Yersinia pestis is a Gram-negative coccobacillus causing the dangerous disease, plague. Survival of Y. pestis within host macrophages is important in the initial stages of infection. In our present work, DNA microarray was used to determine the expression profiles of Y. pestis strain 201 in response to in vitro simulating conditions of Mg²⁺ limitation, polymyxin treatment and oxidative stress that could be found in phagolysosomal environment. It was demonstrated that Y. pestis made appropriate adaptive/protective responses to survive the stressful environments. There are the induced expression of antiphagocytic factors and Mg²⁺ transporters under Mg²⁺ limitation condition, the stimulation of drug/analogue sensitivity and glycerol assimilation after polymyxin treatment, and the differential expression in genes encoding stress-responsive proteins, components of cell envelope, iron assimilation and regulatory functions in response to both Mg²⁺ limitation and polymyxin treatment. Under oxidative stress, Y. pestis uses several mechanisms, especially including the induced expression of detoxification enzymes and DNA repair proteins, to protect from or repair the oxidative cell damages. This microarray analysis would provide the candidates for identifying genes or pathways required for growth and proliferation of Y. pestis in macrophages.