Extraction and antioxidant property of polyhydroxylated naphthoquinone pigments from spines of purple sea urchin Strongylocentrotus nudus

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• 作者：Da-Yong Zhou^a ; Lei Qin^a ; Bei-Wei Zhu^a ; ^{zhubeiwei@163.com"" rel=""nofollow} ; Xiao-Dong Wang^a ; Hui Tan^a ; Jing-Feng Yang^a ; Dong-Mei Li^a ; Xiu-Ping Dong^a ; Hai-Tao Wu^a ; Li-Ming Sun^a ; Xiu-Ling Li^b ; Yoshiyuki Murata^c
• 关键词：Sea urchin ; Natural pigment ; Naphthoquinone ; Extraction ; Antioxidant
• 刊名：Food Chemistry
• 出版年：2011
• 出版时间：15 December 2011
• 年：2011
• 卷：129
• 期：4
• 页码：1591-1597
• 全文大小：358 K

文摘

Purple sea urchin (Strongylocentrotus nudus) spines were dissolved in HCl–ethanol-aqueous solution. The polyhydroxylated 1,4-naphthoquinone (PHNQ) pigments were condensed and purified by using macroporous resin in static adsorption mode. PHNQ in the extract were characterised rapidly by using an ultra-performance liquid chromatography (UPLC) coupled to hybrid quadrupole orthogonal acceleration time-of-flight mass spectrometer (Q-TOFMS). Six known compounds including spinochrome E, 2,7-dihydroxynaphthazarin, spinochrome B, spinochrome C, spinochrome A and echinochrome A, and two new compounds with molecular formula of aminopentahydroxynaphthoquinone and acetylaminotrihydroxynaphthoquinone were identified tentatively. The pigment extract was evaluated for antioxidant activity by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity assay, Fe²⁺ chelating assay, reducing power assay, lipid peroxidation inhibition assay and tertiary-butyl hydroperoxide (t-BOOH)-induced macrophages protection assay. In all testing methods, the extract showed excellent activity, indicating the PHNQ from S. nudus spines are potential sources of natural antioxidants.