Combining Gal4p-mediated expression enhancement and directed evolution of isoprene synthase to improve isoprene production in Saccharomyces cerevisiae

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• 作者：Fan Wang^a ; Xiaomei Lv^a ; Wenping Xie^a ; Pingping Zhou^a ; Yongqiang Zhu^a ; Zhen Yao^a ; Chengcheng Yang^a ; Xiaohong Yang^a ; Lidan Ye^a ; ^b ; ^{yelidan@zju.edu.cn} ; Hongwei Yu^a ; ^{yuhongwei@zju.edu.cn}
• 关键词：Isoprene ; GAL4 regulation ; Directed evolution ; Isoprene synthase ; Saccharomyces cerevisiae
• 刊名：Metabolic Engineering
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：39
• 期：Complete
• 页码：257-266
• 全文大小：932 K
• 卷排序：39

文摘

Strengthening isoprene-forming pathway by improving expression and activity of isoprene synthase. Adoption of GAL4 overexpression for metabolic regulation of isoprene biosynthesis. Directed evolution of isoprene synthase based on DMAPP toxicity. Highest isoprene production ever reported in engineered eukaryotes.