pH-Sensitive Fluorescence Lifetime Molecular Probes Based on Functionalized Tristyrylbenzene

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• 作者：Pedro J. Pacheco-Liñán ; Andrés Garzón ; Juan Tolosa ; Iván Bravo ; Jesús Canales-Vázquez ; Julián Rodríguez-López ; José Albaladejo ; Joaquín C. García-Martínez
• 刊名：Journal of Physical Chemistry C
• 出版年：2016
• 出版时间：August 25, 2016
• 年：2016
• 卷：120
• 期：33
• 页码：18771-18779
• 全文大小：586K
• 年卷期：0
• ISSN：1932-7455

文摘

The dependence of the fluorescence on pH for two 1,3,5-tristyrylbenzenes decorated with polyamine (compound 1) and poly(amidoamine) (compound 2) chains at the periphery was investigated. The highest fluorescence intensities were observed under acidic conditions because electrostatic repulsions between positively charged molecules reduce the fluorescence quenching. The slopes observed in the fluorescence pH titration curves were associated with deprotonation of the different types of amine groups, which results in quenching by photoinduced electron transfer and aggregation processes. The linear dependence of fluorescence lifetime observed for different pH ranges is a valuable property for applications in the field of fluorescence lifetime sensors and imaging microscopy. The influence of the pH and the peripheral chains on the aggregation processes was also analyzed by absorption and emission spectroscopy, dynamic light scattering measurements, and transmission electron microscopy. For compound 1, bands associated with the formation of aggregates were detected along with micrometric aggregates surrounded by fibers with lattice fringes typical of columnar mesophases. For compound 2, which contains longer peripheral chains with a higher degree of branching, aggregates with lower internal order were observed. In this case, the peripheral chains hindered aggregation by π-stacking although the amine groups did allow hydrogen bonding.