Dual-Emitting Quantum Dot/Quantum Rod-Based Nanothermometers with Enhanced Response and Sensitivity in Live Cells

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• 作者：Aaron E. Albers ; Emory M. Chan ; Patrick M. McBride ; Caroline M. Ajo-Franklin ; Bruce E. Cohen ; Brett A. Helms
• 刊名：The Journal of the American Chemical Society
• 出版年：2012
• 出版时间：June 13, 2012
• 年：2012
• 卷：134
• 期：23
• 页码：9565-9568
• 全文大小：269K
• 年卷期：v.134,no.23(June 13, 2012)
• ISSN：1520-5126

文摘

Temperature is a key parameter in physiological processes, and probes able to detect small changes in local temperature are necessary for accurate and quantitative physical descriptions of cellular events. Several have recently emerged that offer excellent temperature sensitivity, spatial resolution, or cellular compatibility, but it has been challenging to realize all of these properties in a single construct. Here, we introduce a luminescent nanocrystal-based sensor that achieves this with a 2.4% change/掳C ratiometric response over physiological temperatures in aqueous buffers, with a precision of at least 0.2 掳C. Thermoresponsive dual emission is conferred by a F枚rster resonant energy transfer (FRET) process between CdSe鈥揅dS quantum dot鈥搎uantum rods (QD鈥換Rs) as donors and cyanine dyes as acceptors, which are conjugated to QD鈥換Rs using an amphiphilic polymer coating. The nanothermometers were delivered to live cells using a pH-responsive cationic polymer colloid, which served to both improve uptake and release nanocrystals from endosomal confinement. Within cells, they showed an unexpected enhancement in their temperature response and sensitivity, highlighting the need to calibrate these and similar probes within the cell.