Recovery and Characterization of the Metal Polymeric Organic Fraction (Polymerin) from Olive Oil Mill Wastewaters
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- 作者：Renato Capasso ; Antonio de Martino ; and Michele Arienzo
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2002
- 出版时间：May 8, 2002
- 年：2002
- 卷：50
- 期：10
- 页码：2846 - 2855
- 全文大小：111K
- 年卷期：v.50,no.10(May 8, 2002)
- ISSN：1520-5118
文摘
A dark and complex metal polymeric organic mixture, named polymerin, was recovered from olive oilmill wastewaters (OMWW) and characterized by chemical analysis, diffuse reflectance infrared Fouriertransform spectroscopy (DRIFTS), and atomic absorption spectrometry (AAS). Polymerin proved tobe composed of carbohydrates (52.40 mg 100-1, w/w), melanin (26.14 mg 100-1), and proteins (10.40mg 100-1), and the respective composition of monosaccharides, phenols, and amino acids wasdetermined. It also contained metals (11.06 mg 100-1), mainly K+ and, to a lesser extent, Na+, Ca2+,Mg2+, Zn2+, Fe3+, and Cu2+, which were naturally bound and chelated through carboxylate anionsand other characteristic nucleophilic functional groups naturally occurring in polymerin. The distributionof polymerin relative molecular size was assessed to be approximately between 500.0 and 2.0 kDaby calibrated molecular weight gel filtration chromatography, indicating also that a fraction consistedof protein, melanin, and polysaccharide, strongly aggregated to each other in a supramolecular statusby a combination of covalent and hydrogen bonds and CH/
interactions, and another fraction ofonly free polysaccharide. Polymerin was transformed into a potassium salt deglycosylated derivative,named KSDpolymerin, which was also characterized by chemical analysis, DRIFTS, and AAS.KSDpolymerin consisted of carbohydrates (6.00 mg 100-1), melanin (52.49 mg 100-1), and proteins(35.40 mg 100-1), and the composition of monosaccharides, phenols, and amino acids wasdetermined. It also contained metals (6.11 mg 100-1), mainly K+ and to a lesser extent Na+, Ca2+,Mg2+, Zn2+ and Fe3+, bound as in polymerin. All the organic components were strongly linked in asupramolecular aggregate status and the relative average molecular size proved to be 6.3 kDa. Finally,we briefly discuss the possible use of such polymerins in agriculture as bioamendments and macro-and microelement biointegrators and as a biofilter for toxic metal removal, in light of their similaritywith humic acids.Keywords: Olea europea; olive oil mill wastewaters; polymers; supramolecular structure; pigments;metals; polysaccharides; proteins; melanin; phenols; chromatography; diffuse reflectance infrared Fouriertransform spectroscopy (DRIFTS); atomic absorption spectrometry (AAS)
interactions, and another fraction ofonly free polysaccharide. Polymerin was transformed into a potassium salt deglycosylated derivative,named KSDpolymerin, which was also characterized by chemical analysis, DRIFTS, and AAS.KSDpolymerin consisted of carbohydrates (6.00 mg 100-1), melanin (52.49 mg 100-1), and proteins(35.40 mg 100-1), and the composition of monosaccharides, phenols, and amino acids wasdetermined. It also contained metals (6.11 mg 100-1), mainly K+ and to a lesser extent Na+, Ca2+,Mg2+, Zn2+ and Fe3+, bound as in polymerin. All the organic components were strongly linked in asupramolecular aggregate status and the relative average molecular size proved to be 6.3 kDa. Finally,we briefly discuss the possible use of such polymerins in agriculture as bioamendments and macro-and microelement biointegrators and as a biofilter for toxic metal removal, in light of their similaritywith humic acids.Keywords: Olea europea; olive oil mill wastewaters; polymers; supramolecular structure; pigments;metals; polysaccharides; proteins; melanin; phenols; chromatography; diffuse reflectance infrared Fouriertransform spectroscopy (DRIFTS); atomic absorption spectrometry (AAS)