Design, Synthesis, and Use of MMP-2 Inhibitor-Conjugated Quantum Dots in Functional Biochemical Assays

详细信息    查看全文

• 作者：Erika Bourguet ; Kristina Brazhnik ; Alyona Sukhanova ; Gautier Moroy ; Sylvie Brassart-Pasco ; Anne-Pascaline Martin ; Isabelle Villena ; Georges Bellon ; Janos Sapi ; Igor Nabiev
• 刊名：Bioconjugate Chemistry
• 出版年：2016
• 出版时间：April 20, 2016
• 年：2016
• 卷：27
• 期：4
• 页码：1067-1081
• 全文大小：808K
• 年卷期：0
• ISSN：1520-4812

文摘

The development of chemically designed matrix metalloprotease (MMP) inhibitors has advanced the understanding of the roles of MMPs in different diseases. Most MMP probes designed are fluorogenic substrates, often suffering from photo- and chemical instability and providing a fluorescence signal of moderate intensity, which is difficult to detect and analyze when dealing with crude biological samples. Here, an inhibitor that inhibits MMP-2 more selectively than Galardin has been synthesized and used for enzyme labeling and detection of the MMP-2 activity. A complete MMP-2 recognition complex consisting of a biotinylated MMP inhibitor tagged with the streptavidin-quantum dot (QD) conjugate has been prepared. This recognition complex, which is characterized by a narrow fluorescence emission spectrum, long fluorescence lifetime, and negligible photobleaching, has been demonstrated to specifically detect MMP-2 in in vitro sandwich-type biochemical assays with sensitivities orders of magnitude higher than those of the existing gold standards employing organic dyes. The approach developed can be used for specific in vitro visualization and testing of MMP-2 in cells and tissues with sensitivities significantly exceeding those of the best existing fluorogenic techniques.