Pores Formation on Cell Membranes by Hederacolchiside A1 Leads to a Rapid Release of Proteins for Cytosolic Subproteome Analysis
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- 作者：Gabriel D. Mazzucchelli ; Nicolas A. Cellier ; Vakhtang Mshviladzade ; Riad Elias ; Yong-Ho Shim ; David Touboul ; Loï ; c Quinton ; Alain Brunelle ; Olivier Lapré ; vote ; Edwin A. De Pauw ; Marie-Claire A.
- 刊名：Journal of Proteome Research
- 出版年：2008
- 出版时间：April 2008
- 年：2008
- 卷：7
- 期：4
- 页码：1683 - 1692
- 全文大小：9113K
- 年卷期：v.7,no.4(April 2008)
- ISSN：1535-3907
文摘
Hederacolchiside A1 was used to progressively permeabilize the membrane of human melanoma MEL-5 cells. Holes formation was followed by Scanning Electron Microscopy and interaction of the saponin with cholesterol and phospholipids by TOF-SIMS. 2D-LC-MS/MS and 2D-SDS-PAGE show that the release of soluble proteins into serum-free culture media increases with time. This can lead to a new rapid and efficient strategy to analyze the cytosolic subproteome and it opens the door to get information from the cytosolic compartment for clinical proteomic studies.