Optimization of Functionalized Polymer Layers for Specific Targeting of Mobile Receptors on Cell Surfaces

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• 作者：Matthew C. Hagy ; Shihu Wang ; Elena E. Dormidontova
• 刊名：Langmuir
• 出版年：2008
• 出版时间：November 18, 2008
• 年：2008
• 卷：24
• 期：22
• 页码：13037-13047
• 全文大小：553K
• 年卷期：v.24,no.22(November 18, 2008)
• ISSN：1520-5827

文摘

The reversible binding between a planar polymer layer functionalized by ligands and a planar cell surface containing different densities of mobile receptors has been studied by Monte Carlo simulations. Using the acceptance-ratio method, the distance-dependent profiles for the average number of ligands bound to receptors, the total free energy for the polymer layer722;cell surface interaction and the interaction force were obtained. Four main design parameters for the polymer layer were considered: the degree of functionalization, chain degree of polymerization, polymer grafting density and the binding energy for the ligand–receptor interaction. We found that an increase in the degree of functionalization or in the absolute energy of ligand722;receptor binding results in a larger number of ligands bound to the receptors, lower free energy, and stronger attractive force. Polymer layers composed of shorter chains were found to exhibit a deeper and narrower free energy profile and a larger attractive force, while longer tethers can interact with the cell surface at a larger and broader range of separation distances, in agreement with experimental observations. Our simulation results show that the increase in polymer grafting density from the mushroom to brush regime enhances the ligand availability and results in a stronger attractive force, increases the maximum binding distance, but exhibits a shallower free energy minimum due to the smaller tolerance to compression for polymer layers with high grafting density. We used two measures of the polymer layer binding affinity to the cell surface: the free energy minimum, related to the equilibrium binding constant and the fraction of bound ligands. We found that the polymer layers with a smaller chain length and grafting density, larger degree of functionalization, and larger absolute binding energy exhibit both a larger equilibrium binding constant to the cell surface and a larger average number of bound ligands, except for high binding energies when the maximum level of binding is reached independently of polymer length and grafting density. We showed that high binding specificity can be achieved by the polymer layers with intermediate ligand722;receptor binding energies or an intermediate number of ligands, as a larger binding energy or number of ligands ensures a high binding affinity but lacks specificity while a smaller binding energy or number of ligands provides inadequate affinity. We found that the results for polymer layers with different properties follow a similar pattern when both high binding affinity to cells with high receptor density and high binding specificity are considered. As a result, the optimal design of the polymer layers can be achieved by using several different strategies, which are discussed.