Structural Elucidation and Structure鈥揂nti-inflammatory Activity Relationships of Cembranoids from Cultured Soft Corals Sinularia sandensis and Sinularia flexibilis

详细信息    查看全文

• 作者：Tsung-Chang Tsai ; Hsueh-Yu Chen ; Jyh-Horng Sheu ; Michael Y. Chiang ; Zhi-Hong Wen ; Chang-Feng Dai ; Jui-Hsin Su
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：August 19, 2015
• 年：2015
• 卷：63
• 期：32
• 页码：7211-7218
• 全文大小：473K
• ISSN：1520-5118

文摘

New cembranoids 4-carbomethoxyl-10-epigyrosanoldie E (1), 7-acetylsinumaximol B (2), diepoxycembrene B (6), dihydromanaarenolide I (8), and isosinulaflexiolide K (9), along with 11 known related metabolites, were isolated from cultured soft corals Sinularia sandensis and Sinularia flexibilis. The structures were elucidated by means of infrared, mass spectrometry, and nuclear magnetic resonance techniques, and the absolute configurations of 1, 4, 9, and 15 were further confirmed by single-crystal X-ray diffraction analysis. The absolute configurations of these coral metabolites and comparison with known analogues showed that one hypothesis (that cembrane diterpenes possessing an absolute configuration of an isopropyl group at C1 obtained from Alcyonacean soft corals belong to the 伪 series, whereas analogues isolated from Gorgonacean corals belong to the 尾 series) is not applicable for a small number of cembranoids. An in vitro anti-inflammatory study using LPS-stimulated macrophage-like cell line RAW 264.7 revealed that compounds 9鈥?4 significantly suppressed the accumulation of pro-inflammatory proteins, iNOS and COX-2. Structure鈥揳ctivity relationship analysis indicated that cembrane-type compounds with one seven-membered lactone moiety at C-1 are potential anti-inflammatory agents. This is the first culture system in the world that has successfully been used to farm S. sandensis.