Endophytic Fungi from Pigeon Pea [Cajanus cajan (L.) Millsp.] Produce Antioxidant Cajaninstilbene Acid
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- 作者：JinTong Zhao ; YuJie Fu ; Meng Luo ; YuanGang Zu ; Wei Wang ; ChunJian Zhao ; ChengBo Gu
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：May 2, 2012
- 年：2012
- 卷：60
- 期：17
- 页码：4314-4319
- 全文大小：296K
- 年卷期：v.60,no.17(May 2, 2012)
- ISSN：1520-5118
文摘
In this study, novel endophytic fungi producing cajaninstilbene acid (CSA) from pigeon pea [Cajanus cajan (L.) Millsp.] were investigated and screened. CSA has prominent pharmacological activities. A total of 110 endophytic fungi isolates were grouped into 8 genera on the basis of morphological characteristics, and CSA-producing fungi were screened by liquid chromatography鈥搕andem mass spectrometry (LC-MS/MS). According to ITS-rDNA sequences analysis, the CSA-producing fungi were identified as Fusarium solani (ERP-07), Fusarium oxysporum (ERP-10), and Fusarium proliferatum (ERP-13), respectively. The amount of CSA produced by the ERP-13 reached 504.8 卤 20.1 渭g/L or 100.5 卤 9.4 渭g/g dry weight of mycelium. In a DPPH radical-scavenging assay, when the concentration of fungal CSA was 500 渭g/mL, inhibition percentage could reach 80%, which was almost the same as that of standard CSA. This study first reported the natural antioxidant CSA from endophytic fungi F. solani and F. proliferatum isolated from pigeon pea.