Sharing Structure and Function in Biological Design with SBOL 2.0

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• 作者：Nicholas Roehner ; Jacob Beal ; Kevin Clancy ; Bryan Bartley ; Goksel Misirli ; Raik Grünberg ; Ernst Oberortner ; Matthew Pocock ; Michael Bissell ; Curtis Madsen ; Tramy Nguyen ; Michael Zhang ; Zhen Zhang ; Zach Zundel ; Douglas Densmore ; John H. Gennari ; Anil Wipat ; Herbert M. Sauro ; Chris J. Myers
• 刊名：ACS Synthetic Biology
• 出版年：2016
• 出版时间：June 17, 2016
• 年：2016
• 卷：5
• 期：6
• 页码：498-506
• 全文大小：496K
• 年卷期：0
• ISSN：2161-5063

文摘

The Synthetic Biology Open Language (SBOL) is a standard that enables collaborative engineering of biological systems across different institutions and tools. SBOL is developed through careful consideration of recent synthetic biology trends, real use cases, and consensus among leading researchers in the field and members of commercial biotechnology enterprises. We demonstrate and discuss how a set of SBOL-enabled software tools can form an integrated, cross-organizational workflow to recapitulate the design of one of the largest published genetic circuits to date, a 4-input AND sensor. This design encompasses the structural components of the system, such as its DNA, RNA, small molecules, and proteins, as well as the interactions between these components that determine the system’s behavior/function. The demonstrated workflow and resulting circuit design illustrate the utility of SBOL 2.0 in automating the exchange of structural and functional specifications for genetic parts, devices, and the biological systems in which they operate.