High-Resolution Mass Spectrometry Associated with Data Mining Tools for the Detection of Pollutants and Chemical Characterization of Honey Samples

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• 作者：J茅r么me Cotton ; Fanny Leroux ; Simon Broudin ; Myl猫ne Marie ; Bruno Corman ; Jean-Claude Tabet ; C茅line Ducruix ; Christophe Junot
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2014
• 出版时间：November 19, 2014
• 年：2014
• 卷：62
• 期：46
• 页码：11335-11345
• 全文大小：490K
• ISSN：1520-5118

文摘

Analytical methods for food control are mainly focused on restricted lists of well-known contaminants. This paper shows that liquid chromatography鈥揾igh-resolution mass spectrometry (LC/ESI-HRMS) associated with the data mining tools developed for metabolomics can address this issue by enabling (i) targeted analyses of pollutants, (ii) detection of untargeted and unknown xenobiotics, and (iii) detection of metabolites useful for the characterization of food matrices. A proof-of-concept study was performed on 76 honey samples. Targeted analysis indicated that 35 of 83 targeted molecules were detected in the 76 honey samples at concentrations below regulatory limits. Furthermore, untargeted metabolomic-like analyses highlighted 12 chlorinated xenobiotics, 1 of which was detected in lavender honey samples and identified as 2,6-dichlorobenzamide, a metabolite of dichlobenil, a pesticide banned in France since 2010. Lastly, multivariate statistical analyses discriminated honey samples according to their floral origin, and six discriminating metabolites were characterized thanks to the MS/MS experiments.

Keywords:

high-resolution mass spectrometry; honey; metabolomics; data mining; metabolite; xenobiotics; pollutants; liquid chromatography; multiresidue; food analysis; electrospray; veterinary drugs; pesticides; bees