New Exposure System To Evaluate the Toxicity of (Scooter) Exhaust Emissions in Lung Cells in Vitro

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• 作者：Loretta Mller ; Pierre Comte ; Jan Czerwinski ; Markus Kasper ; Andreas C. R. Mayer ; Peter Gehr ; Heinz Burtscher ; Jean-Paul Morin ; Athanasios Konstandopoulos ; Barbara Rothen-Rutishauser
• 刊名：Environmental Science & Technology
• 出版年：2010
• 出版时间：April 1, 2010
• 年：2010
• 卷：44
• 期：7
• 页码：2632-2638
• 全文大小：388K
• 年卷期：v.44,no.7(April 1, 2010)
• ISSN：1520-5851

文摘

A constantly growing number of scooters produce an increasing amount of potentially harmful emissions. Due to their engine technology, two-stroke scooters emit huge amounts of adverse substances, which can induce adverse pulmonary and cardiovascular health effects. The aim of this study was to develop a system to expose a characterized triple cell coculture model of the human epithelial airway barrier, to freshly produced and characterized total scooter exhaust emissions. In exposure chambers, cell cultures were exposed for 1 and 2 h to 1:100 diluted exhaust emissions and in the reference chamber to filtered ambient air, both controlled at 5% CO₂, 85% relative humidity, and 37 °C. The postexposure time was 0−24 h. Cytotoxicity, used to validate the exposure system, was significantly increased in exposed cell cultures after 8 h postexposure time. (Pro-) inflammatory chemo- and cytokine concentrations in the medium of exposed cells were significantly higher at the 12 h postexposure time point. It was shown that the described exposure system (with 2 h exposure duration, 8 and 24 h postexposure time, dilution of 1:100, flow of 2 L/min as optimal exposure conditions) can be used to evaluate the toxic potential of total exhaust emissions.