Efforts toward the Expansion of the Genetic Alphabet: Information Storage and Replication with Unnatural Hydrophobic Base Pairs
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- 作者：Anthony K. Ogawa ; Yiqin Wu ; Dustin L. McMinn ; Jianquan Liu ; Peter G. Schultz ; and Floyd E. Romesberg
- 刊名：Journal of the American Chemical Society
- 出版年：2000
- 出版时间：April 12, 2000
- 年：2000
- 卷：122
- 期：14
- 页码：3274 - 3287
- 全文大小：293K
- 年卷期：v.122,no.14(April 12, 2000)
- ISSN：1520-5126
文摘
The faithful recognition of the interstrand hydrogen bonds between complementary nucleobasesforms the foundation of the genetic code. The ability to replicate DNA containing a stable third base pairwould allow for an expansion of the information content of DNA by supplementing the existing two basepairs of the genetic alphabet with a third. We report the optimization of unnatural nucleobases whose pairingin duplex DNA is based on interbase hydrophobic interactions. We show that the stability and selectivity ofsuch unnatural base pairs may be comparable to, or even exceed, that of native pairs. We also demonstratethat several unnatural base pairs are incorporated into DNA by Klenow fragment of Escherichia coli DNApolymerase I with an efficiency equivalent to that of native DNA synthesis. Moreover, the unnatural bases areorthogonal to the native bases, with correct pairing being favored by at least an order of magnitude relative tomispairing.