Live Cell Chemical Profiling of Temporal Redox Dynamics in a Photoautotrophic Cyanobacterium

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• 作者：Natalie C. Sadler ; Matthew R. Melnicki ; Margrethe H. Serres ; Eric D. Merkley ; William B. Chrisler ; Eric A. Hill ; Margaret F. Romine ; Sangtae Kim ; Erika M. Zink ; Suchitra Datta ; Richard D. Smith ; Alexander S. Beliaev ; Allan Konopka ; Aaron T. Wright
• 刊名：ACS Chemical Biology
• 出版年：2014
• 出版时间：January 17, 2014
• 年：2014
• 卷：9
• 期：1
• 页码：291-300
• 全文大小：485K
• ISSN：1554-8937

文摘

Protein reduction鈥搊xidation (redox) modification is an important mechanism that allows microorganisms to sense environmental changes and initiate cellular responses. We have developed a quantitative chemical probe approach for live cell labeling and imaging of proteins that are sensitive to redox modifications. We utilize this in vivo strategy to identify 176 proteins undergoing 5鈥?0-fold dynamic redox change in response to nutrient limitation and subsequent replenishment in the photoautotrophic cyanobacterium Synechococcus sp. PCC 7002. We detect redox changes in as little as 30 s after nutrient perturbation and oscillations in reduction and oxidation for 60 min following the perturbation. Many of the proteins undergoing dynamic redox transformations participate in the major components for the production (photosystems and electron transport chains) or consumption (Calvin鈥揃enson cycle and protein synthesis) of reductant and/or energy in photosynthetic organisms. Thus, our in vivo approach reveals new redox-susceptible proteins and validates those previously identified in vitro.