Cytotoxicity and Gene Expression in Sarcoma 180 Cells in Response to Spiky Magnetoplasmonic Supraparticles

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• 作者：Hongjian Zhou ; Sun Il Choi ; Fengming Zou ; Sangjin Oh ; Ji Eun Kim ; Dae Youn Hwang ; Jaebeom Lee
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2014
• 出版时间：November 26, 2014
• 年：2014
• 卷：6
• 期：22
• 页码：19680-19689
• 全文大小：571K
• ISSN：1944-8252

文摘

Multifunctional nanoparticles (NPs) have been designed for a variety of cell imaging and therapeutic applications, and the study of their cellular interactions is crucial to the development of more efficient biomedical applications. Among current nanomaterials, concave core鈥搒hell NPs with complex angled geometries are attractive owing to their unique shape-dependent optical and physical properties as well as different tendency for cell interaction. In this study, we investigated the morphology effect of spiky gold-coated iron oxide supraparticles (Fe₃O₄@Au SPs) on cytotoxicity and global gene expression in sarcoma 180 cells. Cells treated for 7 days with spiky supraparticles (SPs) at concentrations up to 50 渭g/mL showed >90% viability, indicating that these NPs were nontoxic. To shed light on the differences in cytotoxicity, we monitored the expression of 33鈥?15 genes using microarray analysis of SP-treated cells. The 171 up-regulated genes and 181 down-regulated genes in spiky SP-treated cells included Il1b, Spp1, Il18, Rbp4, and Il11ra1, where these genes are mainly involved in cell proliferation, differentiation, and apoptosis. These results suggested that the spiky Fe₃O₄@Au SPs can induce noncytotoxicity and gene expression in tumor cells, which may be a promising cornerstone on which to base related research such as cyto-/genotoxicology of nanomaterials or the design of nanoscale drug carriers.

Keywords:

concave core鈭抯hell nanoparticle; tumor cell; cytotoxicity; microarray; gene expression