Dual-Mode SERS-Fluorescence Immunoassay Using Graphene Quantum Dot Labeling on One-Dimensional Aligned Magnetoplasmonic Nanoparticles

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• 作者：Fengming Zou ; Hongjian Zhou ; Tran Van Tan ; Jeonghyo Kim ; Kwangnak Koh ; Jaebeom Lee
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：June 10, 2015
• 年：2015
• 卷：7
• 期：22
• 页码：12168-12175
• 全文大小：452K
• ISSN：1944-8252

文摘

A novel dual-mode immunoassay based on surface-enhanced Raman scattering (SERS) and fluorescence was designed using graphene quantum dot (GQD) labels to detect a tuberculosis (TB) antigen, CFP-10, via a newly developed sensing platform of linearly aligned magnetoplasmonic (MagPlas) nanoparticles (NPs). The GQDs were excellent bilabeling materials for simultaneous Raman scattering and photoluminescence (PL). The one-dimensional (1D) alignment of MagPlas NPs simplified the immunoassay process and enabled fast, enhanced signal transduction. With a sandwich-type immunoassay using dual-mode nanoprobes, both SERS signals and fluorescence images were recognized in a highly sensitive and selective manner with a detection limit of 0.0511 pg mL^鈥?.