NopM and NopD Are Rhizobial Nodulation Outer Proteins: Identification Using LC-MALDI and LC-ESI with a Monolithic Capillary Column

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• 作者：Joã ; o A. Rodrigues ; Francisco Javier Ló ; pez-Baena ; Francisco Javier Ollero ; José ; Mar&iacute ; a Vinardell ; Mar&iacute ; a del Rosario Espuny ; Ramon A. Bellog&iacute ; n ; José ; Enriq
• 刊名：Journal of Proteome Research
• 出版年：2007
• 出版时间：March 2007
• 年：2007
• 卷：6
• 期：3
• 页码：1029 - 1037
• 全文大小：366K
• 年卷期：v.6,no.3(March 2007)
• ISSN：1535-3907

文摘

We have explored the potential of commercial polystyrene-divinylbenzene monolithic capillary nanoLC-MS/MS for identifying Sinorhizobium fredii HH103 nodulation outer proteins. Monolithic nanoLC withoff-line MALDI-TOF/TOF and on-line ESI-q-oTOF is fast and robust, generating complementary dataand offering high-confidence protein identifications from gel bands too weak for successful analysisusing traditional approaches. This has allowed identification of two proteins not previously describedas being type III-secreted in rhizobia, NopM and NopD.