Dual Native Chemical Ligation at Lysine

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• 作者：Renliang Yang ; Kalyan Kumar Pasunooti ; Fupeng Li ; Xue-Wei Liu ; Chuan-Fa Liu
• 刊名：Journal of the American Chemical Society
• 出版年：2009
• 出版时间：September 30, 2009
• 年：2009
• 卷：131
• 期：38
• 页码：13592-13593
• 全文大小：147K
• 年卷期：v.131,no.38(September 30, 2009)
• ISSN：1520-5126

文摘

A thiol group introduced on the γ-carbon of lysine mediates robust native chemical ligation at both the α- and ε-amines in two consecutive steps. Desulfurization then affords the final product, in which the lysine residue at the ligation site has an isopeptide bond on its side chain. The method is useful for the synthesis of proteins containing special post-translational modifications on lysine.