Selective Biomolecular Nanoarrays for Parallel Single-Molecule Investigations

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• 作者：Matteo Palma ; Justin J. Abramson ; Alon A. Gorodetsky ; Erika Penzo ; Ruben L. Gonzalez ; Jr. ; Michael P. Sheetz ; Colin Nuckolls ; James Hone ; Shalom J. Wind
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：May 25, 2011
• 年：2011
• 卷：133
• 期：20
• 页码：7656-7659
• 全文大小：766K
• 年卷期：v.133,no.20(May 25, 2011)
• ISSN：1520-5126

文摘

The ability to direct the self-assembly of biomolecules on surfaces with true nanoscale control is key for the creation of functional substrates. Herein we report the fabrication of nanoscale biomolecular arrays via selective self-assembly on nanopatterned surfaces and minimized nonspecific adsorption. We demonstrate that the platform developed allows for the simultaneous screening of specific protein鈥揇NA binding events at the single-molecule level. The strategy presented here is generally applicable and enables high-throughput monitoring of biological activity in real time and with single-molecule resolution.