Determination of the Macromolecular Dimensions of Hydrophobically Modified Polymers by Micellar Size Exclusion Chromatography Coupled With Multiangle Light Scattering

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• 作者：Guillaume Dupuis ; Julien Rigolini ; Grald Clisson ; David Rousseau ; Ren Tabary ; Bruno Grassl
• 刊名：Analytical Chemistry
• 出版年：2009
• 出版时间：November 1, 2009
• 年：2009
• 卷：81
• 期：21
• 页码：8993-9001
• 全文大小：200K
• 年卷期：v.81,no.21(November 1, 2009)
• ISSN：1520-6882

文摘

The present work demonstrates that the use of a nonionic surfactant in the mobile phase together with light scattering coupled to size exclusion chromatography (SEC) provides an accurate determination of macromolecular dimensions of hydrophobically modified water-soluble polymer and polyelectrolyte, i.e., weight-average molar mass M_w and polydispersity I_p. This method, called micellar SEC, is based on the dissociation of the aggregates in aqueous solution and the formation of mixed micelles between the surfactant and the polymer hydrophobic groups. The methodology and its application are presented for synthetic sulfonated polyacrylamides (5 and 20 mol %) modified with three hydrophobic alkyl side groups (C8, C12, and C18) and with Triton X-100 as a nonionic surfactant and are discussed according to the associativity of polymers. The results are compared to those obtained by classical SEC in 0.1 M NaNO₃ and by static light scattering in formamide solution.