Enhancement of Surface Graft Density of MPEG on Alginate/Chitosan Hydrogel Microcapsules for Protein Repellency

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• 作者：Jiani Zheng ; Hongguo Xie ; Weiting Yu ; Mingqian Tan ; Faquan Gong ; Xiudong Liu ; Feng Wang ; Guojun Lv ; Wanfa Liu ; Guoshuang Zheng ; Yan Yang ; Weiyang Xie ; Xiaojun Ma
• 刊名：Langmuir
• 出版年：2012
• 出版时间：September 18, 2012
• 年：2012
• 卷：28
• 期：37
• 页码：13261-13273
• 全文大小：653K
• 年卷期：v.28,no.37(September 18, 2012)
• ISSN：1520-5827

文摘

Alginate/chitosan/alginate (ACA) hydrogel microcapsules were modified with methoxy poly(ethylene glycol) (MPEG) to improve protein repellency and biocompatibility. Increased MPEG surface graft density (n_S) on hydrogel microcapsules was achieved by controlling the grafting parameters including the buffer layer substrate, membrane thickness, and grafting method. X-ray photoelectron spectroscopy (XPS) model was employed to quantitatively analyze n_S on this three-dimensional (3D) hydrogel network structure. Our results indicated that neutralizing with alginate, increasing membrane thickness, and in situ covalent grafting could increase n_S effectively. ACAC_PEG was more promising than ACC_PEG in protein repellency because alginate supplied more 鈭扖OO^{鈥?/sup> negative binding sites and prevented MPEG from diffusing. The n_S increased with membrane thickness, showing better protein repellency. Moreover, the in situ covalent grafting provided an effective way to enhance n_S, and 1.00 卤 0.03 chains/nm2 was achieved, exhibiting almost complete immunity to protein adsorption. This antifouling hydrogel biomaterial is expected to be useful in transplantation in vivo.}