Macroporous Biodegradable Cryogels of Synthetic Poly(伪-amino acids)

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• 作者：Tom谩拧 Sedla膷铆k ; Vladim铆r Proks ; Miroslav 艩louf ; Miroslava Du拧kov谩-Smr膷kov谩 ; Hana Studenovsk谩 ; Franti拧ek Ryp谩膷ek
• 刊名：Biomacromolecules
• 出版年：2015
• 出版时间：November 9, 2015
• 年：2015
• 卷：16
• 期：11
• 页码：3455-3465
• 全文大小：752K
• ISSN：1526-4602

文摘

We present an investigation of the preparation of highly porous hydrogels based on biodegradable synthetic poly(伪-amino acid) as potential tissue engineering scaffolds. Covalently cross-linked gels with permanent pores were formed under cryogenic conditions by free-radical copolymerization of poly[N⁵-(2-hydroxyethyl)-l-glutamine-stat-N⁵-(2-methacryloyl-oxy-ethyl)-l-glutamine] (PHEG-MA) with 2-hydrohyethyl methacrylate (HEMA) and, optionally, N-propargyl acrylamide (PrAAm) as minor comonomers. The morphology of the cryogels showed interconnected polyhedral or laminar pores. The volume content of communicating water-filled pores was >90%. The storage moduli of the swollen cryogels were in the range of 1鈥? kPa, even when the water content was >95%. The enzymatic degradation of a cryogel corresponded to the decrease in its storage modulus during incubation with papain, a model enzyme with specificity analogous to wound-healing enzymes. It was shown that cryogels with incorporated alkyne groups can easily be modified with short synthetic peptides using azide-alkyne cycloaddition 鈥渃lick鈥?chemistry, thus providing porous hydrogel scaffolds with biomimetic features.