Quantum Dots in an Amphiphilic Polyethyleneimine Derivative Platform for Cellular Labeling, Targeting, Gene Delivery, and Ratiometric Oxygen Sensing

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• 作者：Joonhyuck Park ; Junhwa Lee ; Jungheon Kwag ; Yeonggyeong Baek ; Bumju Kim ; Calvin Jinse Yoon ; Seoyeon Bok ; So-Hye Cho ; Ki Hean Kim ; G-One Ahn ; Sungjee Kim
• 刊名：ACS Nano
• 出版年：2015
• 出版时间：June 23, 2015
• 年：2015
• 卷：9
• 期：6
• 页码：6511-6521
• 全文大小：754K
• ISSN：1936-086X

文摘

Amphiphilic polyethyleneimine derivatives (amPEIs) were synthesized and used to encapsulate dozens of quantum dots (QDs). The QD鈥揳mPEI composite was 鈭?00 nm in hydrodynamic diameter and had the slightly positive outer surface that suited well for cellular internalization. The QD鈥揳mPEI showed very efficient QD cellular labeling with the labeled cell fluorescence intensity more than 10 times higher than conventional techniques such as Lipofectamine-assisted QD delivery. QD鈥揳mPEI was optimal for maximal intracellular QD delivery by the large QD payload and the rapid endocytosis kinetics. QD鈥揳mPEI platform technology was demonstrated for gene delivery, cell-specific labeling, and ratiometric oxygen sensing. Our QD鈥揳mPEI platform has two partitions: positive outer surface and hydrophobic inside pocket. The outer positive surface was further exploited for gene delivery and targeting. Co-delivery of QDs and GFP silencing RNAs was successfully demonstrated by assembling siRNAs to the outer surfaces, which showed the transfection efficiency an order of magnitude higher than conventional gene transfections. Hyaluronic acids were tethered onto the QD鈥揳mPEI for cell-specific targeted labeling which showed the specific-to-nonspecific signal ratio over 100. The inside hydrophobic compartment was further applied for cohosting oxygen sensing phosphorescence Ru dyes along with QDs. The QD-Ru-amPEI oxygen probe showed accurate and reversible oxygen sensing capability by the ratiometric photoluminescence signals, which was successfully applied to cellular and spheroid models.