Ultraslow Domain Motions in HIV-1 TAR RNA Revealed by Solid-State Deuterium NMR

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• 作者：Wei Huang ; Prashant S. EmaniGabriele Varani ; Gary P. Drobny
• 刊名：Journal of Physical Chemistry B
• 出版年：2017
• 出版时间：January 12, 2017
• 年：2017
• 卷：121
• 期：1
• 页码：110-117
• 全文大小：502K
• ISSN：1520-5207

文摘

Intrinsic motions may allow HIV-1 transactivation response (TAR) RNA to change its conformation to form a functional complex with the Tat protein, which is essential for viral replication. Understanding the dynamic properties of TAR necessitates determining motion on the intermediate nanosecond-to-microsecond time scale. To this end, we performed solid-state deuterium NMR line-shape and T_1Z relaxation-time experiments to measure intermediate motions for two uridine residues, U40 and U42, within the lower helix of TAR. We infer global motions at rates of ∼10⁵ s^–1 in the lower helix, which are much slower than those in the upper helix (∼10⁶ s^–1), indicating that the two helical domains reorient independently of one another in the solid-state sample. These results contribute to the aim of fully describing the properties of functional motions in TAR RNA.