Improvement of Protein Immobilization and Bioactivity of Magnetic Carriers Using a Brushed Beads-on-Beads Structure

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• 作者：Peirui Wang ; Ping Xu ; Pingping Wang ; Lingling Deng ; Hongchen Gu ; Hong Xu
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：November 11, 2015
• 年：2015
• 卷：7
• 期：44
• 页码：24390-24395
• 全文大小：396K
• ISSN：1944-8252

文摘

To achieve higher protein immobilization and bioactivity, as well as automatic manipulation, we prepared a new type of biocarrier based on the brushed beads-on-beads structure. Many poly(acrylic acid) (PAA) brushed nanoparticles were packed onto the surface of amino-functionalized magnetic particles through an efficient carbodiimide-assisted coupling reaction to attain a hierarchical structure, a unique three-dimensional (3D) space and automatic manipulation characteristics. The proposed biocarrier was evaluated in the recognition capability of the immunocomplex and showed a 6.7-fold increase compared with control beads with a hard surface. The results of this study suggest promising applications in targeted capture and high-performance biodetection processes.