Cross-Linking versus Non-Cross-Linking Aggregation of Gold Nanoparticles Induced by DNA Hybridization: A Comparison of the Rapidity of Solution Color Change

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• 作者：Guoqing Wang ; Yoshitsugu Akiyama ; Shota Shiraishi ; Naoki Kanayama ; Tohru Takarada ; Mizuo Maeda
• 刊名：Bioconjugate Chemistry
• 出版年：2017
• 出版时间：January 18, 2017
• 年：2017
• 卷：28
• 期：1
• 页码：270-277
• 全文大小：524K
• ISSN：1520-4812

文摘

Gold nanoparticles densely modified with single-stranded DNA (ssDNA–AuNPs) form aggregates with cross-linker ssDNAs via duplex formation. Alternatively, the ssDNA–AuNPs are spontaneously aggregated at high ionic strength in a non-cross-linking manner when complementary ssDNAs are added to form fully matched duplexes. Both aggregation modes are accompanied by a red-to-purple color change, which has been exploited in various bioassays. The current study compares the rapidity of color change between the cross-linking and non-cross-linking aggregation modes under identical conditions. When a small number of cross-linker/complementary DNAs are provided, the cross-linking mode exhibited more rapid color change than the non-cross-linking mode. Conversely, with a large number of the DNAs, the non-cross-linking aggregation occurred more rapidly than the cross-linking counterpart. This finding allows one to select a more appropriate aggregation mode for application of ssDNA–AuNPs to colorimetric assays under given conditions.