Regulation of Crosstalk between Epithelial to Mesenchymal Transition Molecules and MMP-9 Mediates the Antimetastatic Activity of Anethole in DU145 Prostate Cancer Cells

详细信息    查看全文

• 作者：ByungChul Ha ; HyunSuk Ko ; BongLee Kim ; Eun Jung Sohn ; Ji Hoon Jung ; Ji Sung Kim ; Jung Jae Yoon ; Gunho Won ; Ji-Hyun Kim ; Deok-beom Jung ; Miyong Yun ; BumSang Shim ; Sung-Hoon Kim
• 刊名：Journal of Natural Products
• 出版年：2014
• 出版时间：January 24, 2014
• 年：2014
• 卷：77
• 期：1
• 页码：63-69
• 全文大小：380K
• ISSN：1520-6025

文摘

The underlying antimetastatic mechanism of anethole (1) still remains unclear in association with the molecules of the epithelial to mesenchymal transition (EMT). Herein, the role of the EMT molecules was elucidated in terms of the antimetastatic activity of 1 using DU145 cells. Anethole significantly inhibited the adhesion of DU145 cells to vitronectin-coated plates, as well as migration in a wound-healing assay and invasion using a Boyden chamber. Also, anethole suppressed the expression of MMP-9 in DU145 cells by zymography, ELISA, and RT-PCR. Consistently, the silencing of MMP-9 enhanced the activity of 1 to upregulate the expression of E-cadherin and to attenuate the expression of Vimentin in DU145 cells. Compound 1 enhanced E-cadherin, which is an epithelial marker and attenuated the expression of Vimentin, Twist, and Snail as mesenchymal molecules at the mRNA level. Consistently, anethole upregulated E-cadherin and downregulated the expression of Vimentin, Twist and PI3K, and AKT at the protein level in DU145 cells. Conversely, the antimetastatic effects of 1 to inhibit invasion and the expression of MMP-9 and upregulate E-cadherin were reversed by the EMT inducer TGF-尾 in DU145 cells. Overall, the present findings suggest that anethole exerts antimetastatic activity via regulation of crosstalk between EMT molecules and MMP-9 on the basis of the in vitro data obtained.