Old Apple (Malus domestica L. Borkh) Varieties with Hypoallergenic Properties: An Integrated Approach for Studying Apple Allergenicity

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• 作者：Mara Vegro ; Giulia Eccher ; Francesca Populin ; Chiara Sorgato ; Federica Savazzini ; Giulia Pagliarani ; Stefano Tartarini ; Gabriella Pasini ; Andrea Curioni ; Andrea Antico ; Alessandro Botton
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：December 7, 2016
• 年：2016
• 卷：64
• 期：48
• 页码：9224-9236
• 全文大小：752K
• ISSN：1520-5118

文摘

Freshly consumed apples (Malus domestica L. Borkh) can cause allergic reactions because of the presence of four classes of allergens. Knowledge of the genetic factors affecting the allergenic potential of apples would provide important information for the selection of hypoallergenic genotypes, which can be combined with the adoption of new agronomical practices to produce fruits with a reduced amount of allergens. In the present research, a multiple analytical approach was adopted to characterize the allergenic potential of 24 apple varieties released at different ages (pre- and post-green revolution). A specific workflow was set up including protein quantification by means of polyclonal antibodies, immunological analyses with sera of allergic subjects, enzymatic assays, clinical assessments on allergic patients, and gene expression assays on fruit samples. Taken as a whole, the results indicate that most of the less allergenic genotypes were found among those deriving from selection processes carried out prior to the so-called “green revolution”.