Analysis of Changes in Gene Expression and Metabolic Profiles Induced by Silica-Coated Magnetic Nanoparticles
详细信息 查看全文
- 作者：Wooyoung Shim ; Man Jeong Paik ; Duc-Toan Nguyen ; Jin-Kyu Lee ; Yuwon Lee ; Jae-Ho Kim ; Eun-Hee Shin ; Jin Seok Kang ; Hak-Sung Jung ; Sangdun Choi ; Sungsu Park ; Jeom Soon Shim ; Gwang Lee
- 刊名：ACS Nano
- 出版年：2012
- 出版时间：September 25, 2012
- 年：2012
- 卷：6
- 期：9
- 页码：7665-7680
- 全文大小：900K
- 年卷期：v.6,no.9(September 25, 2012)
- ISSN：1936-086X
文摘
Magnetic nanoparticles (MNPs) have proven themselves to be useful in biomedical research; however, previous reports were insufficient to address the potential dangers of nanoparticles. Here, we investigated gene expression and metabolic changes based on the microarray and gas chromatography鈥搈ass spectrometry with human embryo kidney 293 cells treated with MNPs@SiO2(RITC), a silica-coated MNP containing Rhodamine B isothiocyanate (RITC). In addition, measurement of reactive oxygen species (ROS) and ATP analysis were performed to evaluate the effect of MNPs@SiO2(RITC) on mitochondrial function. Compared to the nontreated control, glutamic acid was increased by more than 2.0-fold, and expression of genes related to the glutamic acid metabolic pathway was also disturbed in 1.0 渭g/渭L of MNPs@SiO2(RITC)-treated cells. Furthermore, increases in ROS concentration and mitochondrial damage were observed in this MNPs@SiO2(RITC) concentration. The organic acids related to the Krebs cycle were also disturbed, and the capacity of ATP synthesis was decreased in cell treated with an overdose of MNPs@SiO2(RITC). Collectively, these results suggest that overdose (1.0 渭g/渭L) of MNPs caused transcriptomic and metabolic disturbance. In addition, we suggest that a combination of gene expression and metabolic profiles will provide more detailed and sensitive toxicological evaluation for nanoparticles.