Layer-by-Layer Assembly of Supported Lipid Bilayer Poly-l-Lysine Multilayers
详细信息 查看全文
- 作者：George R. Heath ; Mengqiu Li ; Isabelle L. Polignano ; Joanna L. Richens ; Gianluca Catucci ; Paul O’Shea ; Sheila J. Sadeghi ; Gianfranco Gilardi ; Julea N. Butt ; Lars J. C. Jeuken
- 刊名：Biomacromolecules
- 出版年：2016
- 出版时间：January 11, 2016
- 年：2016
- 卷：17
- 期：1
- 页码：324-335
- 全文大小：794K
- ISSN：1526-4602
文摘
Multilayer lipid membranes perform many important functions in biology, such as electrical isolation (myelination of axons), increased surface area for biocatalytic purposes (thylakoid grana and mitochondrial cristae), and sequential processing (golgi cisternae). Here we develop a simple layer-by-layer methodology to form lipid multilayers via vesicle rupture onto existing supported lipid bilayers (SLBs) using poly class="smallcaps">l-lysine (PLL) as an electrostatic polymer linker. The assembly process was monitored at the macroscale by quartz crystal microbalance with dissipation (QCM-D) and the nanoscale by atomic force microscopy (AFM) for up to six lipid bilayers. By varying buffer pH and PLL chain length, we show that longer chains (≥300 kDa) at pH 9.0 form thicker polymer supported multilayers, while at low pH and shorter length PLL, we create close packed layers (average lipid bilayers separations of 2.8 and 0.8 nm, respectively). Fluorescence recovery after photobleaching (FRAP) and AFM were used to show that the diffusion of lipid and three different membrane proteins in the multilayered membranes has little dependence on lipid stack number or separation between membranes. These approaches provide a straightforward route to creating the complex membrane structures that are found throughout nature, allowing possible applications in areas such as energy production and biosensing while developing our understanding of the biological processes at play.