Biothiol Xenon MRI Sensor Based on Thiol-Addition Reaction

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• 作者：Shengjun Yang ; Weiping Jiang ; Lili Ren ; Yaping Yuan ; Bin Zhang ; Qing Luo ; Qianni Guo ; Louis-S. Bouchard ; Maili Liu ; Xin Zhou
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：June 7, 2016
• 年：2016
• 卷：88
• 期：11
• 页码：5835-5840
• 全文大小：406K
• 年卷期：0
• ISSN：1520-6882

文摘

Biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) play an important role in regulating the vital functions of living organisms. Knowledge of their biodistribution in real-time could help diagnose a variety of conditions. However, existing methods of biothiol detection are invasive and require assays. Herein we report a molecular biosensor for biothiol detection using the nuclear spin resonance of ¹²⁹Xe. The ¹²⁹Xe biosensor consists of a cryptophane cage encapsulating a xenon atom and an acrylate group. The latter serves as a reactive site to covalently bond biothiols through a thiol-addition reaction. The biosensor enables discrimination of Cys from Hcy and GSH through the chemical shift and average reaction rate. This biosensor can be detected at a concentration of 10 μM in a single scan and it has been applied to detect biothiols in bovine serum solution. Our results indicate that this biosensor is a promising tool for the real-time imaging of biothiol distributions.