Effects of a New Microbial 伪-Amylase Inhibitor Protein on Helicoverpa armigera Larvae
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- 作者：Fanrong Zeng ; Xiaojing Wang ; Jinjie Cui ; Yan Ma ; Qiannan Li
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2013
- 出版时间：March 6, 2013
- 年：2013
- 卷：61
- 期：9
- 页码：2028-2032
- 全文大小：285K
- 年卷期：v.61,no.9(March 6, 2013)
- ISSN：1520-5118
文摘
A new microbial 伪-amylase inhibitor gene was cloned and characterized. The encoded, recombinant, 伪-amylase inhibitor protein was induced and expressed by isopropyl 尾-d-1-thiogalactopyranoside (IPTG) in Escherichia coli M15 cells. The effects of the 伪-amylase inhibitor protein on Helicoverpa armigera larvae were studied. Compared to the control, the weight of H. armigera larvae fed the diet with recombinant 伪-amylase inhibitor protein added at a concentration of 20 渭g/g was reduced by 49.8%. The total soluble protein of H. armigera larvae fed the diet with the 伪-amylase inhibitor protein added was also reduced by 36.8% compared to the control. The recombinant 伪-amylase inhibitor protein showed inhibition activity against 伪-amylase of H. armigera. These results suggested that this 伪-amylase inhibitor protein may be a promising bioinsecticide candidate for controlling H. armigera.