Quantitative Particle-Induced X-ray Emission Imaging of Rat Olfactory Epithelium Applied to the Permeability of Rat Epithelium to Inhaled Aluminum

详细信息    查看全文

• 作者：Kevin K. Divine ; Johnnye L. Lewis ; Patrick G. Grant ; and Graham Bench
• 刊名：Chemical Research in Toxicology
• 出版年：1999
• 出版时间：July 1999
• 年：1999
• 卷：12
• 期：7
• 页码：575 - 581
• 全文大小：77K
• 年卷期：v.12,no.7(July 1999)
• ISSN：1520-5010

文摘

Neurotoxicity from chronic metal inhalation has been suggested as an underlying contributorto late-developing neurodegenerative diseases that have symptoms similar to Alzheimer's andParkinson's syndromes. If inhaled metals contribute to pathogenesis of these diseases,identifying, localizing, and quantitating metal deposition(s) within specific target regions ofthe central nervous system will be critical to our understanding of the mechanisms. Standardanalytical techniques used to date require exposure to extremely high concentrations of metalsto meet analytical detection limits in small tissue areas. The relevance to lower-doseenvironmentally relevant exposures and potential protective barriers is therefore questionable.The feasibility of microbeam particle-induced X-ray emission is investigated as a method forrapidly scanning tissues to study the inhalation of metals, nasal permeability, and centralnervous system deposition. The optimal beam spot and analysis time used to image the ratolfactory epithelium to facilitate the rapid detection of aluminum localizations were determined.Measurements of aluminum localizations in rat olfactory bulb and brain sections are alsopresented.