Calcium Uncaging with Visible Light

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• 作者：Hitesh K. Agarwal ; Radoslav Janicek ; San-Hui Chi ; Joseph W. Perry ; Ernst Niggli ; Graham C. R. Ellis-Davies
• 刊名：Journal of the American Chemical Society
• 出版年：2016
• 出版时间：March 23, 2016
• 年：2016
• 卷：138
• 期：11
• 页码：3687-3693
• 全文大小：447K
• ISSN：1520-5126

文摘

We have designed a nitroaromatic photochemical protecting group that absorbs visible light in the violet-blue range. The chromophore is a dinitro derivative of bisstyrylthiophene (or BIST) that absorbs light very effectively (ε₄₄₀ = 66,000 M^–1 cm^–1 and two-photon cross section of 350 GM at 775 nm). We developed a “caged calcium” molecule by conjugation of BIST to a Ca²⁺ chelator that upon laser flash photolysis rapidly releases Ca²⁺ in <0.2 ms. Using the patch-clamp method the optical probe, loaded with Ca²⁺, was delivered into acutely isolated mouse cardiac myocytes, where either one- and two-photon uncaging of Ca²⁺ induced highly local or cell-wide physiological Ca²⁺ signaling events.