Thiol-Reactive Molecule with Dual-Emission-Enhancement Property for Specific Prestaining of Cysteine Containing Proteins in SDS-PAGE
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- 作者：Yong Yu ; Jie Li ; Sijie Chen ; Yuning Hong ; Ka Ming Ng ; Kathy Qian Luo ; Ben Zhong Tang
- 刊名：ACS Applied Materials & Interfaces
- 出版年：2013
- 出版时间：June 12, 2013
- 年：2013
- 卷：5
- 期：11
- 页码：4613-4616
- 全文大小：234K
- 年卷期：v.5,no.11(June 12, 2013)
- ISSN：1944-8252
文摘
1-[4-(Bromomethyl)phenyl]-1,2,2-triphenylethene (2) was synthesized and evaluated for specific fluorescent prestaining of proteins containing cysteine (Cys) in SDS-PAGE. The molecule showed classic aggregation-induced emission (AIE) property in protein labeling and its quantum efficiency was further enhanced upon reacting with Cys. The parameters of reaction such as labeling time and concentration of dye and reducing reagent-tris(2-carboxyethyl)phosphine (TCEP) were examined to obtain the optimal labeling condition. In addition to its specific labeling effect, molecule 2 also showed its advantage over traditional self-quenching dyes through labeling Cys containing BSA with different dye/Cys ratios.