Fully Automated Multifunctional Ultrahigh Pressure Liquid Chromatography System for Advanced Proteome Analyses
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- 作者：Jung Hwa Lee ; Seok-Won Hyung ; Dong-Gi Mun ; Hee-Jung Jung ; Hokeun Kim ; Hangyeore Lee ; Su-Jin Kim ; Kyong Soo Park ; Ronald J. Moore ; Richard D. Smith ; Sang-Won Lee
- 刊名：Journal of Proteome Research
- 出版年：2012
- 出版时间：August 3, 2012
- 年：2012
- 卷：11
- 期：8
- 页码：4373-4381
- 全文大小：434K
- 年卷期：v.11,no.8(August 3, 2012)
- ISSN：1535-3907
文摘
A multifunctional liquid chromatography system that performs 1-dimensional, 2-dimensional (strong cation exchange/reverse phase liquid chromatography or SCX/RPLC) separations and online phosphopeptide enrichment using a single binary nanoflow pump has been developed. With a simple operation of a function selection valve equipped with a SCX column and a TiO2 (titanium dioxide) column, a fully automated selection of three different experiment modes was achieved. Because the current system uses essentially the same solvent flow paths, the same trap column, and the same separation column for reverse-phase separation of 1D, 2D, and online phosphopeptides enrichment experiments, the elution time information obtained from these experiments is in excellent agreement, which facilitates correlating peptide information from different experiments. The final reverse-phase separation of the three experiments is completely decoupled from all of the function selection processes; thereby salts or acids from SCX or TiO2 column do not affect the efficiency of the reverse-phase separation.