Label-Free Single-Molecule Imaging with Numerical-Aperture-Shaped Interferometric Scattering Microscopy

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• 作者：Daniel Cole ; Gavin Young ; Alexander Weigel ; Aleksandar Sebesta ; Philipp Kukura
• 刊名：ACS Photonics
• 出版年：2017
• 出版时间：February 15, 2017
• 年：2017
• 卷：4
• 期：2
• 页码：211-216
• 全文大小：536K
• ISSN：2330-4022

文摘

Our ability to optically interrogate nanoscopic objects is controlled by the difference between their extinction cross sections and the diffraction-limited area to which light can be confined in the far field. We show that a partially transmissive spatial mask placed near the back focal plane of a high numerical aperture microscope objective enhances the extinction contrast of a scatterer near an interface by approximately T^–1/2, where T is the transmissivity of the mask. Numerical-aperture-based differentiation of background from scattered light represents a general approach to increasing extinction contrast and enables routine label-free imaging down to the single-molecule level.