Computer-Controlled System for the Study of Oxidase Reactions: Application to the Peroxidase−Oxidase Oscillator

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• 作者：Andrew G. McDonald ; Keith F. Tipton
• 刊名：Journal of Physical Chemistry B
• 出版年：2010
• 出版时间：December 16, 2010
• 年：2010
• 卷：114
• 期：49
• 页码：16244-16252
• 全文大小：358K
• 年卷期：v.114,no.49(December 16, 2010)
• ISSN：1520-5207

文摘

An apparatus for the study of bisubstrate oxidase reactions at maintained steady-state substrate concentrations is described, and its specific application to the peroxidase−oxidase biochemical oscillator is reported. Instrument control and data acquisition are provided by custom software written in LabVIEW. The software allows measurement, recording, and control of dissolved oxygen through a Clark-type oxygen electrode, reaction monitoring by a UV/vis spectrophotometer, and controlled substrate delivery by a syringe infusion pump. For peroxidase from horseradish, the optimal pH for oscillatory behavior was found to be in the range 4.5−5.5.