Cells (MC3T3-E1)-Laden Alginate Scaffolds Fabricated by a Modified Solid-Freeform Fabrication Process Supplemented with an Aerosol Spraying

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• 作者：SeungHyun Ahn ; HyeongJin Lee ; Lawrence J. Bonassar ; GeunHyung Kim
• 刊名：Biomacromolecules
• 出版年：2012
• 出版时间：September 10, 2012
• 年：2012
• 卷：13
• 期：9
• 页码：2997-3003
• 全文大小：520K
• 年卷期：v.13,no.9(September 10, 2012)
• ISSN：1526-4602

文摘

In this study, we propose a new cell encapsulation method consisting of a dispensing method and an aerosol-spraying method. The aerosol spray using a cross-linking agent, calcium chloride (CaCl₂), was used to control the surface gelation of dispensed alginate struts during dispensing. To show the feasibility of the method, we used preosteoblast (MC3T3-E1) cells. By changing the relationship between the various dispensing/aerosol-spraying conditions and cell viability, we could determine the optimal cell-dispensing process: a nozzle size (240 渭m) and an aerosol spray flow rate (0.93 卤 0.12 mL min^鈥?), 10 mm s^鈥? nozzle moving speed, a 10 wt % concentration of CaCl₂ in the aerosol solution, and 2 wt % concentration of CaCl₂ in the second cross-linking process. Based on these optimized process conditions, we successfully fabricated a three-dimensional, pore-structured, cell-laden alginate scaffold of 20 脳 20 脳 4.6 mm³ and 84% cell viability. During long cell culture periods (16, 25, 33, and 45 days), the preosteoblasts in the alginate scaffold survived and proliferated well.