Promoting the Formation and Stabilization of G-Quadruplex by Dinuclear RuII Complex Ru2(obip)L4

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• 作者：Shuo Shi ; Jie Liu ; Tianming Yao ; Xiaoting Geng ; Lingfeng Jiang ; Qingyuan Yang ; Lin Cheng ; Liangnian Ji
• 刊名：Inorganic Chemistry
• 出版年：2008
• 出版时间：April 21, 2008
• 年：2008
• 卷：47
• 期：8
• 页码：2910 - 2912
• 全文大小：341K
• 年卷期：v.47,no.8(April 21, 2008)
• ISSN：1520-510X

文摘

The remarkable ability of a new dinuclear Ru^II complex Ru₂(obip)L₄ [obip = 2-(2-pyridyl)imidazo[4,5-f][1,10]-phenanthroline; L = 2,2′-bipyridine] to promote the formation and stabilization of the human telomeric repeat AG3(T2AG3)3 quadruplex was reported. The experimental results indicated that Ru₂(obip)L₄ could induce the formation of an antiparallel G-quadruplex structure in the absence of metal cations. It could induce positive T_m shifts of +9.4 and +5.8 °C in Na⁺ and K⁺ buffers, respectively, in which an increase in the melting temperature of the quadruplex indicated a stabilizing effect. Binding stoichiometry with the quadruplex was investigated through a luminescence-based Job plot. The major inflection point for Ru₂(obip)L₄ at x = 0.48 was observed. The data were consistent with a 1:1 [quadruplex]/[complex] binding mode, which was suggestive of a specific Ru₂(obip)L₄−quadruplex interaction with a single guanine tetrad.