文摘
In the present work, we describe the properties of a bifunctional redox-labeled bioconjugate at electrode surfacesmediating the electron transfer across the electrode-electrolyte interface. We show that the assembly of ferrocene-labeled streptavidin on biotinylated electrodes results in a reproducible unidirectional current flow in the presenceof electron donors in solution. Such rectifying films were built up by spontaneous binding of tetrameric streptavidinmolecules to biotin centers immobilized on the electrode surface. Due to the high affinitiy of biotin to streptavidin,such bifunctional films completely bind any biotinylated compounds. The charge transport between donors in solutionand the Au electrode is mediated by the ferrocene moieties, allowing us to develop a molecular rectifier. Our experimentalresults suggest that such redox-labeled proteins with a high binding capacity constitute a promising alternative toorganic compounds used in molecular electronics.