Chemopreventive Potential of Powdered Red Wine Pomace Seasonings against Colorectal Cancer in HT-29 Cells

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• 作者：Raquel Del Pino-GarcíaMaría D. Rivero-Pérez ; María L. González-SanJosé ; Miriam Ortega-Heras ; Javier García Lomillo ; Pilar Muñiz
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2017
• 出版时间：January 11, 2017
• 年：2017
• 卷：65
• 期：1
• 页码：66-73
• 全文大小：416K
• ISSN：1520-5118

文摘

This study evaluates the antiproliferative and antigenotoxic actions of powdered red wine pomace seasonings (Sk-S, seedless; W–S, whole; Sd-S, seeds). In vitro gastrointestinal digested and colonic fermented fractions of the seasonings were used as cell treatments. Phenolic acids from Sk-S showed the highest bioaccessibility in the small intestine, whereas polyphenols contained in Sd-S might be the most fermentable in the colon. Dietary fiber from Sk-S was the best substrate for short chain fatty acids production by gut microbiota. Colon cancerous (HT-29) cell viability was inhibited by 50% (IC₅₀ values) at treatment concentrations ranging from 845 (Sk-S) to 1085 (Sd-S) μg/mL prior digestion, but all digested fractions exhibited similar antiproliferative activities (mean IC₅₀ = 814 μg/mL). Oxidative DNA damage in cells was also attenuated by the treatments (200 μg/mL, 24 h preincubation), with all colonic fermented fractions displaying similar genoprotective action. These results suggest the potential of red wine pomace seasonings as chemopreventive agents in colorectal cancer.