Effect of Physical Properties of Nanogel Particles on the Kinetic Constants of Multipoint Protein Recognition Process

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• 作者：Masahiko Nakamoto ; Yu Hoshino ; Yoshiko Miura
• 刊名：Biomacromolecules
• 出版年：2014
• 出版时间：February 10, 2014
• 年：2014
• 卷：15
• 期：2
• 页码：541-547
• 全文大小：407K
• ISSN：1526-4602

文摘

We report the effect of physical properties, such as flexibility and polymer density, of nanogel particles (NPs) on the association/dissociation rates constant (k_on and k_off) and equilibrium constants (K_d) of multipoint protein recognition process. NPs having different flexibilities and densities at 25 掳C were synthesized by tuning cross-linking degrees and the volume phase transition (VPT) temperature. Rate constants were quantified by analyzing time course of protein binding process on NPs monitored by a quartz crystal microbalance (QCM). Both k_on and k_off of swollen phase NPs increased with decreasing cross-linking degree, whereas cross-linking degree did not affect k_on and k_off of the collapsed phase NPs, indicating that polymer density of NPs governs k_on and k_off. The results also suggest that the mechanical flexibility of NPs, defined as the Young鈥檚 modulus, does not always have crucial roles in the multipoint molecular recognition process. On the other hand, K_d was independent of the cross-linking degree and depended only on the phase of NPs, indicating that molecular-scale flexibility, such as side-chain and segmental-mode mobility, as well as the conformation change, of polymer chains assist the formation of stable binding sites in NPs. Our results reveal the rationale for designing NPs having desired affinity and binding kinetics to target molecules.