Applications of Small Molecule Probes in Dissecting Mechanisms of Bacterial Virulence and Host Responses

详细信息    查看全文

• 作者：Aaron W. Puri ; Matthew Bogyo
• 刊名：Biochemistry
• 出版年：2013
• 出版时间：September 3, 2013
• 年：2013
• 卷：52
• 期：35
• 页码：5985-5996
• 全文大小：540K
• 年卷期：v.52,no.35(September 3, 2013)
• ISSN：1520-4995

文摘

Elucidating the molecular and biochemical details of bacterial infections can be challenging because of the many complex interactions that exist between a pathogen and its host. Consequently, many tools have been developed to aid the study of bacterial pathogenesis. Small molecules are a valuable complement to traditional genetic techniques because they can be used to rapidly perturb genetically intractable systems and to monitor post-translationally regulated processes. Activity-based probes are a subset of small molecules that covalently label an enzyme of interest based on its catalytic mechanism. These tools allow monitoring of enzyme activation within the context of a native biological system and can be used to dissect the biochemical details of enzyme function. This review describes the development and application of activity-based probes for examining aspects of bacterial infection on both sides of the host鈥損athogen interface.