Biosynthesis of Monoterpenes and Norisoprenoids in Raspberry Fruits (Rubus idaeus L.): The Role of Cytosolic Mevalonate and Plastidial Methylerythritol Phosphate Pathway

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• 作者：Daniela Hampel ; Anna Swatski ; Armin Mosandl ; Matthias W&#xfc ; st
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2007
• 出版时间：October 31, 2007
• 年：2007
• 卷：55
• 期：22
• 页码：9296 - 9304
• 全文大小：388K
• 年卷期：v.55,no.22(October 31, 2007)
• ISSN：1520-5118

文摘

The biosynthesis of the monoterpenes (−)-α-pinene, linalool, and the norisoprenoids α- and β-ionone in raspberry fruits (rubus idaeus L.) was investigated by in vivo feeding experiments with [5,5-²H₂]-mevalonic acid lactone and [5,5-²H₂]-1-deoxy-D-xylulose. The volatile compounds were extracted by stirbar sorptive extraction and analyzed using thermal desorption-multidimensional gas chromatography–mass spectrometry (TD-enantio-MDGC-MS). The feeding experiments demonstrate that (−)-α-pinene and (S)-linalool are exclusively synthesized via the cytosolic mevalonic acid pathway. In contrast, ²H-labeled (R)-(E)-α-ionone and ²H-labeled (E)-β-ionone are detectable after application of d₂-1-deoxy-D-xylulose and d₂-mevalonic acid lactone, respectively. However, (R)-linalool reveals no incorporation of either one of the fed precursors, even though this enantiomer is detectable in the fruit tissue.