Fate of Fusarium Toxins during the Malting Process

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• 作者：Katharina Habler ; Katharina Hofer ; Cajetan Geißinger ; Jan Schüler ; Ralph Hückelhoven ; Michael Hess ; Martina Gastl ; Michael Rychlik
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：February 17, 2016
• 年：2016
• 卷：64
• 期：6
• 页码：1377-1384
• 全文大小：276K
• ISSN：1520-5118

文摘

Little is known about the fate of Fusarium mycotoxins during the barley malting process. To determine the fungal DNA and mycotoxin concentrations during malting, we used barley grain harvested from field plots that we had inoculated with Fusarium species that produce type A or type B trichothecenes or enniatins. Using a recently developed multimycotoxin liquid chromatography–tandem mass stable isotope dilution method, we identified Fusarium-species-specific behaviors of mycotoxins in grain and malt extracts and compared toxin concentrations to amounts of fungal DNA in the same samples. In particular, the type B trichothecenes and Fusarium culmorum DNA contents were increased dramatically up to 5400% after kilning. By contrast, the concentrations of type A trichothecenes and Fusarium sporotrichioides DNA decreased during the malting process. These data suggest that specific Fusarium species that contaminate the raw grain material might have different impacts on malt quality.