Organelle-Targeted H2S Probes Enable Visualization of the Subcellular Distribution of H2S Donors

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• 作者：Leticia A. Montoya ; Michael D. Pluth
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：June 7, 2016
• 年：2016
• 卷：88
• 期：11
• 页码：5769-5774
• 全文大小：360K
• 年卷期：0
• ISSN：1520-6882

文摘

Hydrogen sulfide (H₂S) is an essential biological signaling molecule in diverse biological regulatory pathways. To provide new chemical tools for H₂S imaging, we report here a fluorescent H₂S detection platform (HSN2-BG) that is compatible with subcellular localization SNAP-tag fusion protein methodologies and use appropriate fusion protein constructs to demonstrate mitochondrial and lysosomal localization. We also demonstrate the efficacy of this detection platform to image endogenous H₂S in Chinese hamster ovary (CHO) cells and use the developed constructs to report on the subcellular H₂S distributions provided by common H₂S donor molecules AP39, ADT–OH, GYY4137, and diallyltrisulfide (DATS). The developed constructs provide a platform poised to provide new insights into the subcellular distribution of common H₂S donors and a useful tool for investigating H₂S biochemistry.