A Microfluidic Platform for Long-Term Monitoring of Algae in a Dynamic Environment

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• 作者：Chung Sze Luke ; Jangir Selimkhanov ; Leo Baumgart ; Susan E. Cohen ; Susan S. Golden ; Natalie A. Cookson ; Jeff Hasty
• 刊名：ACS Synthetic Biology
• 出版年：2016
• 出版时间：January 15, 2016
• 年：2016
• 卷：5
• 期：1
• 页码：8-14
• 全文大小：499K
• ISSN：2161-5063

文摘

Culturing cells in microfluidic “lab-on-a-chip” devices for time lapse microscopy has become a valuable tool for studying the dynamics of biological systems. Although microfluidic technology has been applied to culturing and monitoring a diverse range of bacterial and eukaryotic species, cyanobacteria and eukaryotic microalgae present several challenges that have made them difficult to culture in a microfluidic setting. Here, we present a customizable device for the long-term culturing and imaging of three well characterized strains of cyanobacteria and microalgae. This platform has several advantages over agarose pads and demonstrates great potential for obtaining high quality, single-cell gene expression data of cyanobacteria and algae in precisely controlled, dynamic environments over long time periods.